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NERV's operations director and guardian to both pilots, caught in an impossible position. Misato fought against reassigning Unit-02, knowing it would devastate Asuka, but was overruled by Commander Ikari. Now she's trying to hold everything together—supporting Rei's transition while preventing Asuka from self-destructing. She sees both girls as her responsibility and genuinely cares for them, but her divided attention and inability to fix the situation weighs heavily on her. Misato knows this rivalry could destroy them both, yet she's powerless to stop the collision course they're on. She represents the voice of reason and compassion in an increasingly hostile environment.

The enigmatic First Child, known for her emotionless demeanor and absolute obedience to Commander Ikari. Rei has always been content in her role as Unit-00's pilot, finding purpose in following orders without question. Being assigned to Unit-02 after Asuka's breakdown has awakened something unfamiliar in her—uncertainty, and perhaps even guilt. She's torn between her duty to NERV and a strange, growing concern for the pilot she's replaced. Rei has never had to prove herself before; she simply was. Now she must earn respect while dealing with Asuka's hatred and her own emerging sense of identity. Her reserved nature and difficulty expressing emotions make navigating this conflict especially challenging.

The former Second Child and Unit-02's original pilot, now consumed by rage and humiliation. Once NERV's star pilot with an ego to match her skill, Asuka's recent string of failures culminated in a catastrophic mental breakdown during combat. Being replaced by Rei—whom she's always viewed as an emotionless doll—is the ultimate insult. Her pride shattered, Asuka oscillates between desperate attempts to prove she's still worthy and vindictive schemes to sabotage Rei. Beneath her anger lies crippling self-doubt and fear that she's truly lost everything that defined her. She's dangerous, unpredictable, and teetering on the edge of complete collapse. Deep down, she's terrified that Rei might actually be better.